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neutralizing antibodies against trail  (Takeda)

 
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    Takeda neutralizing antibodies against trail
    Neutralizing Antibodies Against Trail, supplied by Takeda, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/neutralizing+antibodies+against+trail/pm30178306-108-4-25?v=Takeda
    Average 90 stars, based on 1 article reviews
    neutralizing antibodies against trail - by Bioz Stars, 2026-06
    90/100 stars

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    neutralizing antibodies against trail  (Takeda)
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    Takeda neutralizing antibodies against trail
    Neutralizing Antibodies Against Trail, supplied by Takeda, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/neutralizing+antibodies+against+trail/pm30178306-108-4-25?v=Takeda
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    neutralizing antibodies against trail - by Bioz Stars, 2026-06
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    neutralizing antibodies against trail-r1 (dr4  (R&D Systems)
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    Silencing DcR3 combined <t>TRAIL</t> (100 ng/mL) can increase the expression of DR5. Notes: ( A ) The expression of <t>DR4</t> and DR5 was examined by Western blot. GAPHD was used as a loading control. The differences of DR5 among groups were significant ( P <0.001). ( B ) HepG2 cells pretreated with neutralizing antibodies against DR4 and DR5 for 1 h and treated with TRAIL (100 ng/mL) for 24 h. Apoptosis was analyzed by Annexin-V/PI assay. ( C ) The caspase-8 activity was determined fluorometrically in cell extracts using Ac-IETD-AFC as the fluorogenic substrate. P <0.01. Data in the bar graphs represent the mean ± SD of three repeated experiments. Abbreviations: DcR, decoy receptor; h, hours; PI, propidium iodide; SD, standard deviation; TRAIL, tumor necrosis factor-related apoptosis-inducing ligand.
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    Silencing DcR3 combined <t>TRAIL</t> (100 ng/mL) can increase the expression of DR5. Notes: ( A ) The expression of <t>DR4</t> and DR5 was examined by Western blot. GAPHD was used as a loading control. The differences of DR5 among groups were significant ( P <0.001). ( B ) HepG2 cells pretreated with neutralizing antibodies against DR4 and DR5 for 1 h and treated with TRAIL (100 ng/mL) for 24 h. Apoptosis was analyzed by Annexin-V/PI assay. ( C ) The caspase-8 activity was determined fluorometrically in cell extracts using Ac-IETD-AFC as the fluorogenic substrate. P <0.01. Data in the bar graphs represent the mean ± SD of three repeated experiments. Abbreviations: DcR, decoy receptor; h, hours; PI, propidium iodide; SD, standard deviation; TRAIL, tumor necrosis factor-related apoptosis-inducing ligand.
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    Silencing DcR3 combined <t>TRAIL</t> (100 ng/mL) can increase the expression of DR5. Notes: ( A ) The expression of <t>DR4</t> and DR5 was examined by Western blot. GAPHD was used as a loading control. The differences of DR5 among groups were significant ( P <0.001). ( B ) HepG2 cells pretreated with neutralizing antibodies against DR4 and DR5 for 1 h and treated with TRAIL (100 ng/mL) for 24 h. Apoptosis was analyzed by Annexin-V/PI assay. ( C ) The caspase-8 activity was determined fluorometrically in cell extracts using Ac-IETD-AFC as the fluorogenic substrate. P <0.01. Data in the bar graphs represent the mean ± SD of three repeated experiments. Abbreviations: DcR, decoy receptor; h, hours; PI, propidium iodide; SD, standard deviation; TRAIL, tumor necrosis factor-related apoptosis-inducing ligand.
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    neutralizing monoclonal antibodies against trail  (Takeda)
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    Silencing DcR3 combined <t>TRAIL</t> (100 ng/mL) can increase the expression of DR5. Notes: ( A ) The expression of <t>DR4</t> and DR5 was examined by Western blot. GAPHD was used as a loading control. The differences of DR5 among groups were significant ( P <0.001). ( B ) HepG2 cells pretreated with neutralizing antibodies against DR4 and DR5 for 1 h and treated with TRAIL (100 ng/mL) for 24 h. Apoptosis was analyzed by Annexin-V/PI assay. ( C ) The caspase-8 activity was determined fluorometrically in cell extracts using Ac-IETD-AFC as the fluorogenic substrate. P <0.01. Data in the bar graphs represent the mean ± SD of three repeated experiments. Abbreviations: DcR, decoy receptor; h, hours; PI, propidium iodide; SD, standard deviation; TRAIL, tumor necrosis factor-related apoptosis-inducing ligand.
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    neutralizing antibody against apo2l/trail  (Immunex Corporation)
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    Silencing DcR3 combined <t>TRAIL</t> (100 ng/mL) can increase the expression of DR5. Notes: ( A ) The expression of <t>DR4</t> and DR5 was examined by Western blot. GAPHD was used as a loading control. The differences of DR5 among groups were significant ( P <0.001). ( B ) HepG2 cells pretreated with neutralizing antibodies against DR4 and DR5 for 1 h and treated with TRAIL (100 ng/mL) for 24 h. Apoptosis was analyzed by Annexin-V/PI assay. ( C ) The caspase-8 activity was determined fluorometrically in cell extracts using Ac-IETD-AFC as the fluorogenic substrate. P <0.01. Data in the bar graphs represent the mean ± SD of three repeated experiments. Abbreviations: DcR, decoy receptor; h, hours; PI, propidium iodide; SD, standard deviation; TRAIL, tumor necrosis factor-related apoptosis-inducing ligand.
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    Silencing DcR3 combined TRAIL (100 ng/mL) can increase the expression of DR5. Notes: ( A ) The expression of DR4 and DR5 was examined by Western blot. GAPHD was used as a loading control. The differences of DR5 among groups were significant ( P <0.001). ( B ) HepG2 cells pretreated with neutralizing antibodies against DR4 and DR5 for 1 h and treated with TRAIL (100 ng/mL) for 24 h. Apoptosis was analyzed by Annexin-V/PI assay. ( C ) The caspase-8 activity was determined fluorometrically in cell extracts using Ac-IETD-AFC as the fluorogenic substrate. P <0.01. Data in the bar graphs represent the mean ± SD of three repeated experiments. Abbreviations: DcR, decoy receptor; h, hours; PI, propidium iodide; SD, standard deviation; TRAIL, tumor necrosis factor-related apoptosis-inducing ligand.

    Journal: OncoTargets and therapy

    Article Title: Downregulation of DcR3 sensitizes hepatocellular carcinoma cells to TRAIL-induced apoptosis

    doi: 10.2147/OTT.S127202

    Figure Lengend Snippet: Silencing DcR3 combined TRAIL (100 ng/mL) can increase the expression of DR5. Notes: ( A ) The expression of DR4 and DR5 was examined by Western blot. GAPHD was used as a loading control. The differences of DR5 among groups were significant ( P <0.001). ( B ) HepG2 cells pretreated with neutralizing antibodies against DR4 and DR5 for 1 h and treated with TRAIL (100 ng/mL) for 24 h. Apoptosis was analyzed by Annexin-V/PI assay. ( C ) The caspase-8 activity was determined fluorometrically in cell extracts using Ac-IETD-AFC as the fluorogenic substrate. P <0.01. Data in the bar graphs represent the mean ± SD of three repeated experiments. Abbreviations: DcR, decoy receptor; h, hours; PI, propidium iodide; SD, standard deviation; TRAIL, tumor necrosis factor-related apoptosis-inducing ligand.

    Article Snippet: DcR3 polyclonal antibody, recombinant human TRAIL (rhTRAIL), and neutralizing antibodies against TRAIL-R1 (DR4) and TRAIL-R2 (DR5) were purchased from R&D Systems (Minneapolis, MN, USA).

    Techniques: Expressing, Western Blot, Activity Assay, Standard Deviation